diff --git a/Marlin/src/feature/leds/neopixel.h b/Marlin/src/feature/leds/neopixel.h
index 09a5cf8b3..f8300c4d9 100644
--- a/Marlin/src/feature/leds/neopixel.h
+++ b/Marlin/src/feature/leds/neopixel.h
@@ -110,7 +110,7 @@ public:
       #if CONJOINED_NEOPIXEL
         adaneo2.show();
       #else
-        TERN_(NEOPIXEL2_SEPARATE,,adaneo1.setPin(NEOPIXEL2_PIN));
+        TERN_(NEOPIXEL2_SEPARATE,adaneo1.setPin(NEOPIXEL2_PIN));
         adaneo1.show();
         adaneo1.setPin(NEOPIXEL_PIN);
       #endif