diff --git a/Marlin/src/inc/SanityCheck.h b/Marlin/src/inc/SanityCheck.h
index 443669bb2..498455e59 100644
--- a/Marlin/src/inc/SanityCheck.h
+++ b/Marlin/src/inc/SanityCheck.h
@@ -2294,6 +2294,20 @@ static_assert(hbm[Z_AXIS] >= 0, "HOMING_BUMP_MM.Z must be greater than or equal
   #error "GRAPHICAL_TFT_UPSCALE must be set to 2 or 3."
 #endif
 
+/**
+ * Some boards forbid the use of -1 Native USB
+ */
+#if ENABLED(BOARD_NO_NATIVE_USB)
+  #undef BOARD_NO_NATIVE_USB
+  #if SERIAL_PORT == -1
+    #error "SERIAL_PORT is set to -1, but the MOTHERBOARD has no native USB support. Set SERIAL_PORT to a valid value for your board."
+  #elif SERIAL_PORT_2 == -1
+    #error "SERIAL_PORT_2 is set to -1, but the MOTHERBOARD has no native USB support. Set SERIAL_PORT_2 to a valid value for your board."
+  #elif LCD_SERIAL_PORT == -1
+    #error "LCD_SERIAL_PORT is set to -1, but the MOTHERBOARD has no native USB support. Set LCD_SERIAL_PORT to a valid value for your board."
+  #endif
+#endif
+
 /**
  * Serial displays require a dedicated serial port
  */
diff --git a/Marlin/src/pins/stm32f1/pins_CHITU3D.h b/Marlin/src/pins/stm32f1/pins_CHITU3D.h
index 1d8f4f684..aa83a0cb0 100644
--- a/Marlin/src/pins/stm32f1/pins_CHITU3D.h
+++ b/Marlin/src/pins/stm32f1/pins_CHITU3D.h
@@ -21,8 +21,8 @@
  */
 #pragma once
 
-#if NOT_TARGET(__STM32F1__, __STM32F4__)
-  #error "Oops! Select an STM32F1/4 board in 'Tools > Board.'"
+#if NOT_TARGET(__STM32F1__)
+  #error "Oops! Select an STM32F1 board in 'Tools > Board.'"
 #endif
 
 /**
@@ -32,6 +32,8 @@
 #define BOARD_INFO_NAME      "Chitu3D"
 #define DEFAULT_MACHINE_NAME "STM32F103RET6"
 
+#define BOARD_NO_NATIVE_USB
+
 // Enable I2C_EEPROM for testing
 //#define I2C_EEPROM
 
diff --git a/Marlin/src/pins/stm32f1/pins_CHITU3D_V5.h b/Marlin/src/pins/stm32f1/pins_CHITU3D_V5.h
index 181872737..b13fde026 100644
--- a/Marlin/src/pins/stm32f1/pins_CHITU3D_V5.h
+++ b/Marlin/src/pins/stm32f1/pins_CHITU3D_V5.h
@@ -21,8 +21,8 @@
  */
 #pragma once
 
-#if NOT_TARGET(__STM32F1__, __STM32F4__)
-  #error "Oops! Select an STM32F1/4 board in 'Tools > Board.'"
+#if NOT_TARGET(__STM32F1__)
+  #error "Oops! Select an STM32F1 board in 'Tools > Board.'"
 #endif
 
 /**
@@ -32,6 +32,8 @@
 #define BOARD_INFO_NAME      "Chitu3D V5"
 #define DEFAULT_MACHINE_NAME "STM32F103ZET6"
 
+#define BOARD_NO_NATIVE_USB
+
 #define DISABLE_JTAG
 
 //
diff --git a/Marlin/src/pins/stm32f1/pins_CHITU3D_V6.h b/Marlin/src/pins/stm32f1/pins_CHITU3D_V6.h
index 1d6e6b398..a015fb270 100644
--- a/Marlin/src/pins/stm32f1/pins_CHITU3D_V6.h
+++ b/Marlin/src/pins/stm32f1/pins_CHITU3D_V6.h
@@ -21,8 +21,8 @@
  */
 #pragma once
 
-#if NOT_TARGET(__STM32F1__, __STM32F4__)
-  #error "Oops! Select an STM32F1/4 board in 'Tools > Board.'"
+#if NOT_TARGET(__STM32F1__)
+  #error "Oops! Select an STM32F1 board in 'Tools > Board.'"
 #endif
 
 /**
@@ -32,6 +32,8 @@
 #define BOARD_INFO_NAME      "Chitu3D"
 #define DEFAULT_MACHINE_NAME "STM32F103ZET6"
 
+#define BOARD_NO_NATIVE_USB
+
 #define DISABLE_JTAG
 
 //
diff --git a/Marlin/src/pins/stm32f1/pins_CREALITY_V4.h b/Marlin/src/pins/stm32f1/pins_CREALITY_V4.h
index eb910dd84..c6f2dd8b8 100644
--- a/Marlin/src/pins/stm32f1/pins_CREALITY_V4.h
+++ b/Marlin/src/pins/stm32f1/pins_CREALITY_V4.h
@@ -26,9 +26,7 @@
 
 #if NOT_TARGET(__STM32F1__)
   #error "Oops! Select an STM32F1 board in 'Tools > Board.'"
-#endif
-
-#if HOTENDS > 1 || E_STEPPERS > 1
+#elif HOTENDS > 1 || E_STEPPERS > 1
   #error "CREALITY supports up to 1 hotends / E-steppers. Comment out this line to continue."
 #endif
 
@@ -39,6 +37,8 @@
   #define DEFAULT_MACHINE_NAME "Ender 3 V2"
 #endif
 
+#define BOARD_NO_NATIVE_USB
+
 //
 // EEPROM
 //
diff --git a/Marlin/src/pins/stm32f1/pins_FLSUN_HISPEED.h b/Marlin/src/pins/stm32f1/pins_FLSUN_HISPEED.h
index bf3389492..5f9107244 100644
--- a/Marlin/src/pins/stm32f1/pins_FLSUN_HISPEED.h
+++ b/Marlin/src/pins/stm32f1/pins_FLSUN_HISPEED.h
@@ -38,6 +38,8 @@
 #define BOARD_INFO_NAME      "FLSun HiSpeedV1"
 #define BOARD_WEBSITE_URL    "github.com/Foxies-CSTL"
 
+#define BOARD_NO_NATIVE_USB
+
 //
 // Release PB4 (Y_ENABLE_PIN) from JTAG NRST role
 //
diff --git a/Marlin/src/pins/stm32f1/pins_FLY_MINI.h b/Marlin/src/pins/stm32f1/pins_FLY_MINI.h
index 9bb2cd371..073d6c736 100644
--- a/Marlin/src/pins/stm32f1/pins_FLY_MINI.h
+++ b/Marlin/src/pins/stm32f1/pins_FLY_MINI.h
@@ -21,12 +21,12 @@
  */
 #pragma once
 
-#ifndef __STM32F1__
+#if NOT_TARGET(__STM32F1__)
   #error "Oops! Select an STM32F1 board in 'Tools > Board.'"
 #endif
 
 #define BOARD_INFO_NAME   "FLY_MINI"
-#define BOARD_WEBSITE_URL    "github.com/FLYmaker"
+#define BOARD_WEBSITE_URL "github.com/FLYmaker"
 #define DISABLE_JTAG
 
 //
diff --git a/Marlin/src/pins/stm32f1/pins_FYSETC_AIO_II.h b/Marlin/src/pins/stm32f1/pins_FYSETC_AIO_II.h
index bab6182e4..aebb776b2 100644
--- a/Marlin/src/pins/stm32f1/pins_FYSETC_AIO_II.h
+++ b/Marlin/src/pins/stm32f1/pins_FYSETC_AIO_II.h
@@ -28,6 +28,8 @@
 #define BOARD_INFO_NAME   "FYSETC AIO II"
 #define BOARD_WEBSITE_URL "fysetc.com"
 
+#define BOARD_NO_NATIVE_USB
+
 #define DISABLE_JTAG
 
 #define pins_v2_20190128                          // new pins define
diff --git a/Marlin/src/pins/stm32f1/pins_FYSETC_CHEETAH.h b/Marlin/src/pins/stm32f1/pins_FYSETC_CHEETAH.h
index deca1a054..b8a4ccf77 100644
--- a/Marlin/src/pins/stm32f1/pins_FYSETC_CHEETAH.h
+++ b/Marlin/src/pins/stm32f1/pins_FYSETC_CHEETAH.h
@@ -29,11 +29,12 @@
 
 #define BOARD_INFO_NAME   "FYSETC Cheetah"
 #define BOARD_WEBSITE_URL "fysetc.com"
-// https://github.com/FYSETC/Cheetah
 
 // Ignore temp readings during development.
 //#define BOGUS_TEMPERATURE_GRACE_PERIOD    2000
 
+#define BOARD_NO_NATIVE_USB
+
 #define DISABLE_JTAG
 
 #if EITHER(NO_EEPROM_SELECTED, FLASH_EEPROM_EMULATION)
diff --git a/Marlin/src/pins/stm32f1/pins_GTM32_MINI.h b/Marlin/src/pins/stm32f1/pins_GTM32_MINI.h
index 00c572138..52b857ac0 100644
--- a/Marlin/src/pins/stm32f1/pins_GTM32_MINI.h
+++ b/Marlin/src/pins/stm32f1/pins_GTM32_MINI.h
@@ -33,6 +33,8 @@
 #define BOARD_INFO_NAME      "GTM32 Pro VB"
 #define DEFAULT_MACHINE_NAME "STM32F103VET6"
 
+#define BOARD_NO_NATIVE_USB
+
 //#define DISABLE_DEBUG
 
 //
diff --git a/Marlin/src/pins/stm32f1/pins_GTM32_MINI_A30.h b/Marlin/src/pins/stm32f1/pins_GTM32_MINI_A30.h
index b10748e57..85d0be6d6 100644
--- a/Marlin/src/pins/stm32f1/pins_GTM32_MINI_A30.h
+++ b/Marlin/src/pins/stm32f1/pins_GTM32_MINI_A30.h
@@ -33,6 +33,8 @@
 #define BOARD_INFO_NAME      "GTM32 Pro VB"
 #define DEFAULT_MACHINE_NAME "STM32F103VET6"
 
+#define BOARD_NO_NATIVE_USB
+
 //#define DISABLE_DEBUG
 
 //
diff --git a/Marlin/src/pins/stm32f1/pins_GTM32_PRO_VB.h b/Marlin/src/pins/stm32f1/pins_GTM32_PRO_VB.h
index 00c572138..52b857ac0 100644
--- a/Marlin/src/pins/stm32f1/pins_GTM32_PRO_VB.h
+++ b/Marlin/src/pins/stm32f1/pins_GTM32_PRO_VB.h
@@ -33,6 +33,8 @@
 #define BOARD_INFO_NAME      "GTM32 Pro VB"
 #define DEFAULT_MACHINE_NAME "STM32F103VET6"
 
+#define BOARD_NO_NATIVE_USB
+
 //#define DISABLE_DEBUG
 
 //
diff --git a/Marlin/src/pins/stm32f1/pins_GTM32_REV_B.h b/Marlin/src/pins/stm32f1/pins_GTM32_REV_B.h
index b913530f6..292119ce0 100644
--- a/Marlin/src/pins/stm32f1/pins_GTM32_REV_B.h
+++ b/Marlin/src/pins/stm32f1/pins_GTM32_REV_B.h
@@ -33,6 +33,8 @@
 #define BOARD_INFO_NAME      "GTM32 Pro VB"
 #define DEFAULT_MACHINE_NAME "M201"
 
+#define BOARD_NO_NATIVE_USB
+
 //#define DISABLE_DEBUG
 
 //
diff --git a/Marlin/src/pins/stm32f1/pins_JGAURORA_A5S_A1.h b/Marlin/src/pins/stm32f1/pins_JGAURORA_A5S_A1.h
index 0dd8fb69a..f86acf1f2 100644
--- a/Marlin/src/pins/stm32f1/pins_JGAURORA_A5S_A1.h
+++ b/Marlin/src/pins/stm32f1/pins_JGAURORA_A5S_A1.h
@@ -33,8 +33,11 @@
 #elif HOTENDS > 1 || E_STEPPERS > 1
   #error "JGAurora 32-bit board only supports 1 hotend / E-stepper. Comment out this line to continue."
 #endif
+
 #define BOARD_INFO_NAME "JGAurora A5S A1 board"
 
+#define BOARD_NO_NATIVE_USB
+
 #ifndef STM32_XL_DENSITY
   #define STM32_XL_DENSITY
 #endif
diff --git a/Marlin/src/pins/stm32f1/pins_LONGER3D_LK.h b/Marlin/src/pins/stm32f1/pins_LONGER3D_LK.h
index c68557070..fd5c57387 100644
--- a/Marlin/src/pins/stm32f1/pins_LONGER3D_LK.h
+++ b/Marlin/src/pins/stm32f1/pins_LONGER3D_LK.h
@@ -31,6 +31,8 @@
 #define BOARD_INFO_NAME "Longer3D"
 #define ALFAWISE_UX0                              // Common to all Longer3D STM32F1 boards (used for Open drain mosfets)
 
+#define BOARD_NO_NATIVE_USB
+
 //#define DISABLE_DEBUG                           //  We still want to debug with STLINK...
 #define DISABLE_JTAG                              //  We free the jtag pins (PA15) but keep STLINK
                                                   //  Release PB4 (STEP_X_PIN) from JTAG NRST role.
diff --git a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN.h b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN.h
index e6c8098f2..581dd5375 100644
--- a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN.h
+++ b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN.h
@@ -35,6 +35,8 @@
 
 #define BOARD_INFO_NAME "MKS Robin"
 
+#define BOARD_NO_NATIVE_USB
+
 //
 // Release PB4 (Y_ENABLE_PIN) from JTAG NRST role
 //
diff --git a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_E3P.h b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_E3P.h
index 2b7417c29..9c0f2deea 100644
--- a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_E3P.h
+++ b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_E3P.h
@@ -35,6 +35,8 @@
 
 #define BOARD_INFO_NAME "MKS Robin e3p"
 
+#define BOARD_NO_NATIVE_USB
+
 //
 // Release PB4 (Y_ENABLE_PIN) from JTAG NRST role
 //
diff --git a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_E3_common.h b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_E3_common.h
index ffcdc3cfd..2dcd7780a 100644
--- a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_E3_common.h
+++ b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_E3_common.h
@@ -29,6 +29,8 @@
   #error "Oops! Select an STM32F1 board in 'Tools > Board.'"
 #endif
 
+#define BOARD_NO_NATIVE_USB
+
 #define BOARD_WEBSITE_URL "github.com/makerbase-mks"
 
 //#define DISABLE_DEBUG
diff --git a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_LITE.h b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_LITE.h
index ab5350e4d..d7c4aebe3 100644
--- a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_LITE.h
+++ b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_LITE.h
@@ -32,6 +32,8 @@
 #endif
 #define BOARD_WEBSITE_URL "github.com/makerbase-mks"
 
+#define BOARD_NO_NATIVE_USB
+
 //#define DISABLE_DEBUG
 #define DISABLE_JTAG
 
diff --git a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_LITE3.h b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_LITE3.h
index 4c1299fe0..f8b8a891b 100644
--- a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_LITE3.h
+++ b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_LITE3.h
@@ -36,6 +36,8 @@
 #endif
 #define BOARD_WEBSITE_URL "github.com/makerbase-mks"
 
+#define BOARD_NO_NATIVE_USB
+
 //#define DISABLE_DEBUG
 #define DISABLE_JTAG
 
diff --git a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_MINI.h b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_MINI.h
index 8c63f7973..2406c22d5 100644
--- a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_MINI.h
+++ b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_MINI.h
@@ -33,6 +33,8 @@
 
 #define BOARD_INFO_NAME "MKS Robin Mini"
 
+#define BOARD_NO_NATIVE_USB
+
 //
 // Release PB4 (Y_ENABLE_PIN) from JTAG NRST role
 //
diff --git a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_NANO.h b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_NANO.h
index e7e583a6f..3c9b2f5b5 100644
--- a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_NANO.h
+++ b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_NANO.h
@@ -33,6 +33,8 @@
 
 #define BOARD_INFO_NAME "MKS Robin Nano"
 
+#define BOARD_NO_NATIVE_USB
+
 //
 // Release PB4 (Y_ENABLE_PIN) from JTAG NRST role
 //
diff --git a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_NANO_V2.h b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_NANO_V2.h
index e009d34d9..9c1d073da 100644
--- a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_NANO_V2.h
+++ b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_NANO_V2.h
@@ -35,6 +35,8 @@
 
 #define BOARD_INFO_NAME "MKS Robin nano V2.0"
 
+#define BOARD_NO_NATIVE_USB
+
 //
 // Release PB4 (Y_ENABLE_PIN) from JTAG NRST role
 //
diff --git a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_PRO.h b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_PRO.h
index 639a8f057..8f1a405e8 100644
--- a/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_PRO.h
+++ b/Marlin/src/pins/stm32f1/pins_MKS_ROBIN_PRO.h
@@ -33,6 +33,8 @@
 
 #define BOARD_INFO_NAME "MKS Robin pro"
 
+#define BOARD_NO_NATIVE_USB
+
 //
 // Release PB4 (Y_ENABLE_PIN) from JTAG NRST role
 //
diff --git a/Marlin/src/pins/stm32f1/pins_STM3R_MINI.h b/Marlin/src/pins/stm32f1/pins_STM3R_MINI.h
index 3ebc4bca5..ef82b71f5 100644
--- a/Marlin/src/pins/stm32f1/pins_STM3R_MINI.h
+++ b/Marlin/src/pins/stm32f1/pins_STM3R_MINI.h
@@ -21,8 +21,8 @@
  */
 #pragma once
 
-#if NOT_TARGET(__STM32F1__, __STM32F4__)
-  #error "Oops! Select an STM32F1/4 board in 'Tools > Board.'"
+#if NOT_TARGET(__STM32F1__)
+  #error "Oops! Select an STM32F1 board in 'Tools > Board.'"
 #endif
 
 /**
diff --git a/Marlin/src/pins/stm32f1/pins_TRIGORILLA_PRO.h b/Marlin/src/pins/stm32f1/pins_TRIGORILLA_PRO.h
index 8314858d1..2fc34ce29 100644
--- a/Marlin/src/pins/stm32f1/pins_TRIGORILLA_PRO.h
+++ b/Marlin/src/pins/stm32f1/pins_TRIGORILLA_PRO.h
@@ -35,6 +35,9 @@
 #endif
 
 #define BOARD_INFO_NAME "Trigorilla Pro"
+
+#define BOARD_NO_NATIVE_USB
+
 #define DISABLE_JTAG
 
 //
diff --git a/buildroot/tests/mks_robin_lite-tests b/buildroot/tests/mks_robin_lite-tests
index 34809641e..d54627e06 100644
--- a/buildroot/tests/mks_robin_lite-tests
+++ b/buildroot/tests/mks_robin_lite-tests
@@ -8,7 +8,7 @@ set -e
 
 restore_configs
 opt_set MOTHERBOARD BOARD_MKS_ROBIN_LITE
-opt_set SERIAL_PORT -1
+opt_set SERIAL_PORT 1
 opt_enable EEPROM_SETTINGS
 opt_enable SDSUPPORT
 exec_test $1 $2 "Default Configuration with Fallback SD EEPROM"
diff --git a/buildroot/tests/mks_robin_pro-tests b/buildroot/tests/mks_robin_pro-tests
index 50ec7b0dd..3cafc6142 100644
--- a/buildroot/tests/mks_robin_pro-tests
+++ b/buildroot/tests/mks_robin_pro-tests
@@ -8,6 +8,8 @@ set -e
 
 use_example_configs Mks/Robin_Pro
 opt_enable EMERGENCY_PARSER
+opt_set SERIAL_PORT 3
+opt_disable SERIAL_PORT_2
 opt_set SDCARD_CONNECTION LCD
 opt_set X_DRIVER_TYPE TMC2209
 opt_set Y_DRIVER_TYPE TMC2130